Journal: Molecular Medicine Reports

Article Title: The anticancer effects of cinobufagin on hepatocellular carcinoma Huh-7 cells are associated with activation of the p73 signaling pathway

doi: 10.3892/mmr.2019.10108

Figure Lengend Snippet: Cinobufagin may induce anticancer effects on Huh-7 cells via activation of p73 signaling. (A) Protein expression levels of p73, p-p73 (Y99), MDM2, p-MDM2 (S166), p21, Puma and Noxa in cells in cells following treatment with 5 µmol/l cinobufagin for 24 h, as determined by western blotting. Densitometric analysis of (B) p-p73, (C) Puma, (D) p-MDM2, (E) Noxa and (F) p21. (G) Expression of p73 in Huh-7 cells, as determined by immunocytochemistry. Representative images are shown at ×200 magnification. Data are presented as the means ± standard error of the mean of three independent experiments. *P<0.05 vs. control, # P<0.05 vs. cinobufagin. AURKA, aurora kinase A; MDM2, mouse double minute 2 homolog; Noxa, phorbol-12-myristate-13-acetate-induced protein 1; p, phosphorylated; Puma, p53 upregulated modulator of apoptosis.

Article Snippet: The rabbit monoclonal anti-AURKA (cat. no. 14475), rabbit polyclonal anti-p-p73 (Y99; cat. no. 4665) and anti-p-MDM2 (S166; cat. no. 3521) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Activation Assay, Expressing, Western Blot, Immunocytochemistry, Control

Journal: Molecular Medicine Reports

Article Title: The anticancer effects of cinobufagin on hepatocellular carcinoma Huh-7 cells are associated with activation of the p73 signaling pathway

doi: 10.3892/mmr.2019.10108

Figure Lengend Snippet: Schematic diagram of cinobufagin-induced effects on hepatocellular carcinoma Huh-7 cells with mutant p53. Cinobufagin inhibits the viability, arrests the cell cycle and induces the apoptosis of Huh-7 cells by inhibiting AURKA and p53 signaling, and activating p73 signaling. AURKA, aurora kinase A; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; CDK1, cyclin-dependent kinase 1; hnRNPK, heterogeneous nuclear ribonucleoprotein K; NKA, Na + /K + -ATPase; Noxa, phorbol-12-myristate-13-acetate-induced protein 1; p, phosphorylated; PCNA, proliferating cell nuclear antigen; Puma, p53 upregulated modulator of apoptosis.

Article Snippet: The rabbit monoclonal anti-AURKA (cat. no. 14475), rabbit polyclonal anti-p-p73 (Y99; cat. no. 4665) and anti-p-MDM2 (S166; cat. no. 3521) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Mutagenesis